Ústav technické a experimentální fyziky Institute of Experimental and Applied Physics

4D embryogenesis study using X-Ray micro-CT: the pilot experiment

NázevTitle
4D embryogenesis study using X-Ray micro-CT: the pilot experiment4D embryogenesis study using X-Ray micro-CT: the pilot experiment
Druh výsledkuResult type
Příspěvek ve sborníkuProceedings paper
AutořiAuthors
K. Merglová, J. Žemlička, J. Dudák
Časopis / citaceJournal / citation
In: INSTRUMENTS AND METHODS FOR BIOLOGY AND MEDICINE 2018 Conference Proceedings. Prague: Czech Technical University, 2018. p. 41-44. ISBN 978-80-01-06502-0.
JazykLanguage
eng
RIVRIV
RIV/68407700:21460/18:00327474!RIV19-MSM-21460___
ProjektProject
Inženýrské aplikace fyziky mikrosvětaEngineering applications of microworld physics

AbstraktAbstract

The pilot study deals with the use of X-ray micro-CT to monitor the embryonic development of mammals on laboratory mice specimens. As a measuring system we are using the micro-CT system built at the IEAP CTU in Prague. Its construction utilizing nano-focal source and cone beam geometry enables measurements with a spatial resolution smaller than 1 μm. Suitable methods for stabilizing and contrasting the samples were selected on the basis of pilot radiography and tomography measurements. The contribution presents the data obtained by measurement the reference P1 stage of mice stabilized by ascending alcohol line and contrasted with iodine tincture.

The pilot study deals with the use of X-ray micro-CT to monitor the embryonic development of mammals on laboratory mice specimens. As a measuring system we are using the micro-CT system built at the IEAP CTU in Prague. Its construction utilizing nano-focal source and cone beam geometry enables measurements with a spatial resolution smaller than 1 μm. Suitable methods for stabilizing and contrasting the samples were selected on the basis of pilot radiography and tomography measurements. The contribution presents the data obtained by measurement the reference P1 stage of mice stabilized by ascending alcohol line and contrasted with iodine tincture.